MAPTrix¢â Bioadhesive Technology
Mussel adhesive protein (MAP) is a remarkable protein-based adhesive material for adherence to a wide range of the substrates under water. Its unusually strong adhesive and cohesive properties are attributable to dihydroxyphenylalanine (DOPA) residues, which can form crosslinks and bond to surfaces.

As a result of Kollodis' successful development of scaled-up processes for MAP manufacture, Kollodis BioSciences provides recombinant mussel adhesive protein (MAP) products as stand-alone adhesives (bioadhesives) and specialized wet adhesives kit (coacervate coating kit) at economic price.
Stand-alone Adhesive Technology
MAPTrix¢â Adhesive is a lyophilized mussel adhesive protein powder that can be used as a coating to efficiently immobilize or bind biological samples such as tissues, cells, or biomolecules such as proteins to a wide variety of substrates whose surface is hydrophilic or hydrophobic, in wet environment.

MAPTrix¢â Adhesive can produce an even and uniform coating layer with strong adhesion between a substrate and biological samples even under water. It can simplify the manipulation of biological samples in a number of common in vitro techniques, including:
  • Establishment of primary cultures
  • In situ hybridization
  • Immunoassays
Complex Coacervate for Medical Adhesive
Having demonstrated mussel adhesive protein can adhere animal tissues with its little toxicity, its potential as medical adhesive is actually unlimited. However, the mussel adhesive formula should meet the general requirement for medical applications.

Generally, medical adhesive should bond to tissue in a physiological condition that is wet, bloody, ionically rich environments. In addition, the adhesive formulation should be conveniently delivered to wet tissue where it is sufficiently cohesive that the adhesive does not mix with body fluid including water. It should be readily adhere to wet surface with short setting time on a time scale of several minutes.

Complex coacervation was proposed to play a role in the formation of the underwater bioadhesive, and it is believed that mussel secrets its adhesive protein as a complex coacervate, a dense and phase-separated fluid of condensed polymer that is formed from two oppositely charged molecules in an aqueous solution.

Scientists have developed mussel adhesive protein based complex coacervate to mimic nature-inspired underwater adhesive, demonstrating the potential as medical adhersive.

However, the coacervated musse adhesive proteins were too viscous to work with conveniently according to our internal investigation.

The viscosity or flow behavior is an important factor for adhesives based on complex coacervates. For medical applications, the viscosity should be sufficiently high at low shear rates that the adhesive does not flow away from the application site, and yet low enough at high shear rates that it can be conveniently applied through a narrow gauge cannula, or catheter, without high pressure.

Kollodis BioSciences has recently developed practical injectable mussel adhesive protein based complex coacervate to commercialize a wet adhesive. Our goal is to develop tailored adhesive formula with controllable viscosity for wide range of medical applications.
MAPTrix¢â Hydrogel Adhesive
Hydrogels have recently attracted a lot of attention for regenerative medicine due to their innate structural and compositional similarities to the extracellular matrix and their extensive framework for cellular proliferation and survival.

Extracellular microenvironmental factors such as multiple physical, chemical, and biological cues are known to act cooperatively and/or synergistically to affect cellular function during tissue regeneration in vivo. Therefore, hydrogel can be used?as a scaffold capable of controlling cell function and tissue development?by providing synthetic extracellular microenvironments.

MAPTrix¢â HyGel has demonstrated to regulate cellular behavior such as cell growth and morphogenesis in primary HUVEC cell culture (in vitro) by presenting combination of MAPTrix¢â ECM that induces combinatorial integrin-mediated signaling to control cell functions. For this reason, we believe MAPTrix¢â HyGel based adhesive has a great potential as a scaffold providing synthetic extracellular microenvironment to regulate cell function and guided tissue development.

In animal model, MAPTrix¢â HyGel demonstrated good adhesiveness, efficacy for wound healing, no scar formation as well as little immunogenecity.

MAPTrix¢â HyGel has been pre-clinically developed for wound healing for chronic wound repair in collaboration with a pharmaceutical company and a medical school in Korea.

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